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mouse anti calprotectin  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse anti calprotectin
    Mouse Anti Calprotectin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+calprotectin/Calgranulin+A%2FB+Antibody/us12448421-669-20-25
    Average 92 stars, based on 3 article reviews
    mouse anti calprotectin - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Non-transgene transfection for therapeutic purposes
    Article Snippet: .. After blocking one hour with 1% BSA in PBS/0.1% Tween 20 and rinsing 3× with PBS, cells were incubated with mouse anti-calprotectin (mAb 27E10, sc-33714, Santa Cruz Biotechnolology, Inc., Santa Cruz, CA) or mouse anti-LL37 (sc-166770, Santa Cruz Biotechnolology, Inc., Santa Cruz, CA) for one hour, followed by Alexa Fluor 568-conjugated goat anti-mouse IgG or Alexa Fluor 488-conjugated goat anti-rabbit IgG (Life Technologies Corp., Carlsbad, CA) for one hour. ..

    Article Title: Non-transgene transfection for therapeutic purposes
    Article Snippet: .. After blocking one hour with 1% BSA in PBS/0.1% Tween 20 and rinsing 3× with PBS, cells were incubated with mouse anti-calprotectin (mAb 27E10, sc-33714, Santa Cruz Biotechnolology, Inc., Santa Cruz, CA) or mouse anti-LL37 (sc-166770, Santa Cruz Biotechnolology, Inc., Santa Cruz, CA) for one hour, followed by Alexa Fluor 568-conjugated goat anti-mouse IgG or Alexa Fluor 488-conjugated goat anti-rabbit IgG (Life Technologies Corp., Carlsbad, CA) for one hour. ..

    Incubation:

    Article Title: Non-transgene transfection for therapeutic purposes
    Article Snippet: .. After blocking one hour with 1% BSA in PBS/0.1% Tween 20 and rinsing 3× with PBS, cells were incubated with mouse anti-calprotectin (mAb 27E10, sc-33714, Santa Cruz Biotechnolology, Inc., Santa Cruz, CA) or mouse anti-LL37 (sc-166770, Santa Cruz Biotechnolology, Inc., Santa Cruz, CA) for one hour, followed by Alexa Fluor 568-conjugated goat anti-mouse IgG or Alexa Fluor 488-conjugated goat anti-rabbit IgG (Life Technologies Corp., Carlsbad, CA) for one hour. ..

    Article Title: Non-transgene transfection for therapeutic purposes
    Article Snippet: .. After blocking one hour with 1% BSA in PBS/0.1% Tween 20 and rinsing 3× with PBS, cells were incubated with mouse anti-calprotectin (mAb 27E10, sc-33714, Santa Cruz Biotechnolology, Inc., Santa Cruz, CA) or mouse anti-LL37 (sc-166770, Santa Cruz Biotechnolology, Inc., Santa Cruz, CA) for one hour, followed by Alexa Fluor 568-conjugated goat anti-mouse IgG or Alexa Fluor 488-conjugated goat anti-rabbit IgG (Life Technologies Corp., Carlsbad, CA) for one hour. ..



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    Image Search Results


    Detection of NETs formed in vitro. The figure reports the data obtained with increasing amounts of NETs from neutrophils stimulated by PMA or A23187, expressed as DNA concentration. The results are given as absorbance obtained in the MPO−DNA assay ( A ), enolase−DNA assay ( B ) and calprotectin−DNA assay ( C ).

    Journal: International Journal of Molecular Sciences

    Article Title: Novel Methods for the Analysis of Serum NET Remnants: Evaluation in Patients with Severe COVID-19

    doi: 10.3390/ijms26052221

    Figure Lengend Snippet: Detection of NETs formed in vitro. The figure reports the data obtained with increasing amounts of NETs from neutrophils stimulated by PMA or A23187, expressed as DNA concentration. The results are given as absorbance obtained in the MPO−DNA assay ( A ), enolase−DNA assay ( B ) and calprotectin−DNA assay ( C ).

    Article Snippet: For the detection of calprotectin–DNA complexes, plates were coated overnight at 4 °C with 5 μg/mL of mouse anti-human calprotectin monoclonal antibody (Eurospital, Trieste, Italy) diluted in 0.1 M carbonate buffer, pH 9.6.

    Techniques: In Vitro, Concentration Assay

    Levels of NET remnants and correlations among the three assays. Healthy subjects (HS) and COVID-19 sera were tested for MPO−DNA ( A ), enolase−DNA ( B ) and calprotectin−DNA ( C ) complexes. Correlations between MPO−DNA and enolase−DNA ( D ) and between MPO−DNA and calprotectin−DNA ( E ) were also observed. Data are expressed as percentages relative to an internal positive control.

    Journal: International Journal of Molecular Sciences

    Article Title: Novel Methods for the Analysis of Serum NET Remnants: Evaluation in Patients with Severe COVID-19

    doi: 10.3390/ijms26052221

    Figure Lengend Snippet: Levels of NET remnants and correlations among the three assays. Healthy subjects (HS) and COVID-19 sera were tested for MPO−DNA ( A ), enolase−DNA ( B ) and calprotectin−DNA ( C ) complexes. Correlations between MPO−DNA and enolase−DNA ( D ) and between MPO−DNA and calprotectin−DNA ( E ) were also observed. Data are expressed as percentages relative to an internal positive control.

    Article Snippet: For the detection of calprotectin–DNA complexes, plates were coated overnight at 4 °C with 5 μg/mL of mouse anti-human calprotectin monoclonal antibody (Eurospital, Trieste, Italy) diluted in 0.1 M carbonate buffer, pH 9.6.

    Techniques: Positive Control

    NET remnants and clinical parameters. The figure reports the correlations between neutrophil number and MPO−DNA ( A ), enolase−DNA ( C ) and calprotectin−DNA ( E ) complexes and between FiO 2 /PaO 2 and MPO−DNA ( B ), enolase−DNA ( D ) and calprotectin−DNA ( F ) complexes.

    Journal: International Journal of Molecular Sciences

    Article Title: Novel Methods for the Analysis of Serum NET Remnants: Evaluation in Patients with Severe COVID-19

    doi: 10.3390/ijms26052221

    Figure Lengend Snippet: NET remnants and clinical parameters. The figure reports the correlations between neutrophil number and MPO−DNA ( A ), enolase−DNA ( C ) and calprotectin−DNA ( E ) complexes and between FiO 2 /PaO 2 and MPO−DNA ( B ), enolase−DNA ( D ) and calprotectin−DNA ( F ) complexes.

    Article Snippet: For the detection of calprotectin–DNA complexes, plates were coated overnight at 4 °C with 5 μg/mL of mouse anti-human calprotectin monoclonal antibody (Eurospital, Trieste, Italy) diluted in 0.1 M carbonate buffer, pH 9.6.

    Techniques:

    Correlations between clinical parameters and NET remnants. (n.s: not significant).

    Journal: International Journal of Molecular Sciences

    Article Title: Novel Methods for the Analysis of Serum NET Remnants: Evaluation in Patients with Severe COVID-19

    doi: 10.3390/ijms26052221

    Figure Lengend Snippet: Correlations between clinical parameters and NET remnants. (n.s: not significant).

    Article Snippet: For the detection of calprotectin–DNA complexes, plates were coated overnight at 4 °C with 5 μg/mL of mouse anti-human calprotectin monoclonal antibody (Eurospital, Trieste, Italy) diluted in 0.1 M carbonate buffer, pH 9.6.

    Techniques: